Determination of limonoate and nomilinoate A-ring lactones in citrus juices by liquid chromatography-electrospray ionization mass spectrometry.
نویسندگان
چکیده
The development of delayed bitterness in citrus products is a major problem to citrus producers and juice processors worldwide. A rapid and sensitive liquid chromatography-electrospray ionization mass spectrometry (LC-ESI-MS) method has been developed to quantify the recognized precursors of limonoid derived delayed bitterness, limonoate and nomilinoate A-ring lactones, in a wide variety of citrus juices. The limonoid A-ring lactones were isolated by solid-phase extraction from juice samples, analyzed by negative ion LC-ESI-MS and quantified utilizing the standard addition method.
منابع مشابه
Isolation and characterization of limonoate and nomilinoate A-ring lactones.
A method combining solid-phase extraction and reversed-phase high-performance liquid chromatography is described for the isolation of two key metabolites in the limonoid biosynthetic pathway critical to citrus quality. Potassium salts of limonoate A-ring lactone and nomilinoate A-ring lactone were isolated from young Chandler pummelo seedlings and characterized on the basis of proton and carbon...
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ورودعنوان ژورنال:
- Journal of chromatography. A
دوره 1064 2 شماره
صفحات -
تاریخ انتشار 2005